Is this natural?→ ”Master of pronouncing「あいうえお」,will soon be master of the pronouncing Japanese.”
I want it to be a title of my education video.

See a translation

Do you mean, "Mastering the pronunciation of「あいうえお」will make you the master of pronouncing Japanese"

"Master" is a noun, meaning a person. "Master of pronouncing" or "Master of pronunciation" sounds like a "person who the best at pronouncing". (because of the "of")

"Mastering the pronunciation"- is similar to "If you can properly pronounce ( あいうえお)"

The phrase can also be written as-
"If you master the pronouncing of「あいうえお」, you will soon master pronouncing Japanese"

If you mean to say that if a person becomes the master in pronouncing「あいうえお」, then he will become the master of pronouncing Japanese. (Master as a noun).

Then- "If you become the master of pronouncing「あいうえお」, then you will soon become the master of pronouncing Japanese."

There are other ways to paraphrase this I'd end up confusing you. ahaha.

Hope this helped!